Fig. 3: Ablation of ET-1 or Ednrb in the SVZ increases neurogenesis.
From: Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone
a Experimental strategy. b Coronal sections of YFP+ cells in WT, ET-1 cKO, and Ednrb cKO SVZ at P10. Scale bars = 25 μm. c Quantification of the YFP+ cell lineage in the SVZ at P10 (BLBP: n = 3 WT, 4 ET-1 cKO, 4 Ednrb cKO mice. S100β: n = 3 WT, 3 ET-1 cKO, 4 Ednrb cKO mice. Dcx: n = 4 WT, 3 ET-1 cKO, 3 Ednrb cKO mice. Olig2: n = 4 WT, 4 ET-1 cKO, 3 Ednrb cKO mice.). ***p value = 0.0009 (BLBP expression between WT and ET-1 cKO); ***p value = 0.0004 (BLBP expression between WT and Ednrb cKO); **p value = 0.0072 (Dcx expression between WT and ET-1 cKO); ***p value = 0.003 (Dcx expression between WT and Ednrb cKO); *p value = 0.0390 (Dcx expression between ET-1 cKO and Ednrb cKO), n.s. not significant (one-way ANOVA with Tukey’s multiple comparisons test). d Coronal sections of WT, ET-1 cKO, and Ednrb cKO SVZ at P10, staining for different NPC populations. Scale bars = 25 μm. e Quantification of NPC populations in the dorsal SVZ at P10 (Pax6: n = 5 WT, 4 ET-1 cKO, 3 Ednrb cKO mice. Gsx2: n = 5 mice all groups. Sp8: n = 5 mice all groups.). **p value = 0.0090; ***p value = 0.0003 (one-way ANOVA with Tukey’s multiple comparisons test). f Experimental strategy. g Sagittal sections of YFP+ cells in olfactory bulb (OB) of WT, ET-1 cKO, and Ednrb cKO mice at P28. Scale bars = 25 μm. h Quantification of total YFP+ NeuN+ cells per area in OB (n = 5 WT, 4 ET-1 cKO, and 3 Ednrb cKO mice). *p value = 0.0163; **p value = 0.0079 (one-way ANOVA with Tukey’s multiple comparisons test). i Magnified images of YFP+ neurons in OB of WT, ET-1 cKO, and Ednb cKO mice that are negative for Caspase3 staining. Images are representative of 5 WT, 4 ET-1 cKO, and 4 Ednrb cKO mice. Scale bar = 10 μm. LV lateral ventricle. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.
