Fig. 4: ET-1 regulates radial glial proliferation via Notch activation.
From: Endothelin-1 signaling maintains glial progenitor proliferation in the postnatal subventricular zone
a Experimental procedure. b Heatmap depicting significant differentially expressed genes (DEGs) between control and ET-1-treated neurospheres. c Quantification of read counts of stem and progenitor genes that were significantly altered by ET-1 treatment (n = 4 independent batches). d Quantification of read counts of Notch pathway genes that were significantly altered by ET-1 treatment (n = 4 independent batches). e QPCR for Notch pathway components 6 h after ET-1 treatment (n = 3 independent batches). *p value = 0.036405; **p value = 0.001039 (Multiple t-tests with Holm–Sidak multiple comparisons correction). f QPCR for Notch pathway components in SVZ 6 h after stereotaxic injection of Ednrb antagonist BQ788 injection into LVs of P2 WT mice (Jag1: n = 3 saline and 4 BQ788 mice. Notch1: n = 4 saline and 7 BQ788 mice. Hes5: n = 5 saline and 7 BQ788 mice.). *p value = 0.048951 (Multiple t-tests with Holm–Sidak multiple comparisons correction). g QPCR for Notch pathway components in SVZ of WT and ET-1 cKO pups at P7 (n = 3 WT, 6 ET-1 cKO mice). **p value = 0.003901 (Multiple t-tests with Holm–Sidak multiple comparisons correction). Data are presented as mean values ± SEM. Source data are provided as a Source Data file.
