Fig. 6: Identification of downstream molecular targets of ET-1 signaling in OPCs.

a Experimental procedure. b Volcano plot displaying differentially expressed genes (DEGs) between control and ET-1-treated OPCs. The y-axis corresponds to the mean expression value of log10 (p value) and the x-axis displays the log2 fold change value. The red dots represent the transcripts that have a minimum log2 fold change of 0.05 and an adjusted p value of <0.1 (Wald test with Benjamini−Hochberg post hoc). c Predicted upstream regulators from IPA analysis of DEGs that are highlighted in red in b. Yellow arrow points to Edn1 as a predicted upstream regulator. d Venn diagram displaying the number of shared and distinct DEGs identified from the neurosphere and OPC RNAseq datasets. e Heatmap of the 37 DEGs shared by the neurosphere and OPC RNAseq datasets. The heatmap depicts the read counts from the OPC RNAseq data. Only 1 gene (Sgk1*) exhibited the opposite change in the neurosphere RNAseq dataset. f Heatmap of the 41 DEGs specific to the OPC RNAseq dataset. g Representative images of Yfp+ recombined OPCs in the dorsal SVZ of WT and Ednrb OPC-cKO mice at P10. Arrows point to the presence of S100b, Ust, or Gsx1 mRNA puncta within the Yfp+ cells. Scale bar = 10 μm. h Quantification of the percentage of Yfp+ OPCs that contain S100b, Ust, or Gsx1+ mRNA puncta within the dorsal SVZ of WT and Ednrb cKO mice at P10. (n = 3 WT, 3 Ednrb OPC-cKO mice). **p value = 0.007214 (S100b and Gsx1); **p value = 0.006335 (Ust) (Multiple t-tests with Holm–Sidak multiple comparisons correction). Data are presented as mean values ± SEM. Source data are provided as a Source Data file.