Fig. 4: Experimental setups to investigate the effect of various restraints. | Nature Communications

Fig. 4: Experimental setups to investigate the effect of various restraints.

From: Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism

Fig. 4

Three experimental setups with decreasing restraints on the docking geometry of LPHN3 and TEN2 during their interaction. a Setup for trans-cellular interaction of full-length TEN2 with full-length LPHN3 in cell-aggregation experiments. Both proteins are anchored on the cell-membranes and their mobility is restricted by their lateral diffusion within the membrane. b Setup for cis-like-interaction of full-length TEN2 and soluble biotinylated Lec domain of LPHN3 in either flow cytometry or cell-surface staining experiments. TEN2 is anchored on the cell-membrane, but the Lec domain of LPHN3 freely rotates in solution. c Setup for in-solution experiments for cis-like-interaction of soluble TEN2 and soluble LPHN3 in the absence of any membranes.

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