Fig. 4: In vivo transport routes for mutated LBR constructs. | Nature Communications

Fig. 4: In vivo transport routes for mutated LBR constructs.

From: Nucleoplasmic signals promote directed transmembrane protein import simultaneously via multiple channels of nuclear pores

Fig. 4: In vivo transport routes for mutated LBR constructs.

a–d Transport routes of N-terminally tagged LBR Δ63-172, LBR R74T, Lap2β ΔNLS, and LBR ΔLinker respectively. On the left, schematic cartoon representation of each INM protein (orientation N-terminus to the left and C-terminus to the right). The depicted membrane represents a single bilayer of the NE. On the right, 3D probability density map generated by using the 2D to 3D transformation algorithm52, with regions of highest to lowest density (red to yellow in color bar). e Bioinformatic analysis of the fraction of confirmed INM proteins (red, n = 46) and ONM proteins (blue, n = 11) that have a strong NLS sequence, an ID region longer than 65 amino acids, and both of those characteristics arranged in a way that is feasible for transport through the facilitated transport route using both central and peripheral channels of the NPC.

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