Fig. 7: Impaired dendritic cell activation in LTβRTEC mice.
From: Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells
Flow cytometric analysis of CD86 and MHCII expression by Lin−CD11c+Sirpα− cDC1 and Lin−CD11c+Sirpα+ cDC2 in the thymuses of a Foxn1Cre (n = 10 biologically independent samples, closed symbols) and LTβRTEC (n = 11 biologically independent samples, open symbols), and b WT (n = 7 biologically independent samples, closed symbols) Cd1d1−/− (n = 7 biologically independent samples, open symbols). In both cases, graphs in lower panels show quantitation of MHCIIhiCD86hi cDC1 and cDC2 (gated as in upper panels). All data are from three independent experiments. Significant P values using two-tailed unpaired t test are as follows: a cDC1 P = 0.0018, cDC2 P = 0.0014, b cDC1 P = 0.0001, cDC2 P = 0.0004. All data are represented as mean ± SEM. *** P < 0.001 and **P < 0.01. Source data are provided as a Source Data file.
