Fig. 3: Swd2 enhances the Set1–CTD Y2H interaction.
From: The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS
Gal4 BD fused to full length Set1 (Set1) or amino acids 1–236 (Set1–F1, see Fig. 2) were expressed in Y2H strain PJ69-4A carrying wild-type (SWD2) or deletion (swd2Δ) alleles of SWD2. They were combined with a Gal4 AD fusion to the Rpb1 CTD (CTD) or the parent vector (Empty). Cells were spotted on the indicated selective plates. Source data are provided as a Source data file.
