Fig. 6: Effect of caspase inhibitor on in vivo replication and transmission of ZIKV.
From: Zika virus noncoding RNA suppresses apoptosis and is required for virus transmission by mosquitoes
Mosquitoes were i.t. injected with 200 nl of inoculums containing 104 FFU/ml of each virus either with 625 μM of pan-caspase inhibitor Z-VAD-FMK or with vehicle control (NC). a ZIKV titres in mosquito bodies at 14 days after co-injection of viral inoculum with Z-VAD-FMK or vehicle control (NC). b Viral titres in saliva samples collected from caspase inhibitor-treated and untreated ZIKV-infected mosquitoes. c ZIKV transmission rate of WT and sf-RNA-deficient ZIKV by mosquitoes treated with Z-VAD-FMK or vehicle control (NC) determined as a percentage of ZIKV-positive saliva samples (darker shading on the graphs). All titres are determined by IPA on C6/36 cells. Statistical analysis is by independent Mann–Whitney U-tests (no multiple comparisons) (a, b) and chi-squared test (c). All P-values are two-sided, graphs in (a) and (b) show individual and median (horizontal lines) values, sample sizes (n) are indicated in the figure. NC, negative control (vehicle).
