Fig. 1: Effects of PKBα/β deletion in the heart on cardiac function and calcium transients in primary cardiomyocytes.
a PKBα, β and γ protein expression in the heart of male PKBαf/f;MCM;PKBβ−/− mice at 8 weeks after tamoxifen induction. b–e Ejection fraction (EF) (b), fractional shortening (FS) (c), systolic left ventricular volume (LV Vol;s) (d), and diastolic left ventricular volume (LV Vol;d) (e) were measured via echocardiography in the male PKBαf/f; PKBβ−/− and PKBαf/f;MCM;PKBβ−/− mice before and after tamoxifen induction. n = 14 (0 week), 12 (2 week), 12 (4 week), and 12 (6 week) for PKBαf/f;PKBβ−/− mice. n = 12 (0 week), 9 (2 week), 8 (4 week), and 7 (6 week) for PKBαf/f;MCM;PKBβ−/− mice. p = 0.147 (0 week), 1.60e-7 (2 week), 2.15e-8 (4 week) and 5.33e-10 (6 week) for EF. p = 0.165 (0 week), 8.56e-8 (2 week), 1.16e-8 (4 week), and 3.03e-10 (6 week) for FS. p = 0.229 (0 week), 1.05e-6 (2 week), 9.84e-7 (4 week), and 6.36e-8 (6 week) for LV Vol;s. p = 0.387 (0 week), 9.36e-5 (2 week), 2.70e-5 (4 week), and 1.23e-6 (6 week) for LV Vol;d. f Calcium transients elicited by electrical stimulation in primary cardiomyocytes isolated from the male PKBαf/f;PKBβ−/− and PKBαf/f;MCM;PKBβ−/− mice at 4 weeks after tamoxifen induction. Quantitation of amplitude, full duration at half maximum (FDHM) and time constant Tau of calcium transients was shown. 76 cells from 3 PKBαf/f;PKBβ−/− mice and 34 cells from 2 PKBαf/f;MCM;PKBβ−/− mice were analyzed. p = 0.446 (amplitude), 5.96e-5 (FDHM), and 3.99e-3 (tau). The data are given as the mean ± SEM. Statistical analyses for b–f were carried out using two-sided t-test. Two-asterisk indicates p < 0.01, and three-asterisk indicates p < 0.001. Source data are provided as a Source Data file.
