Fig. 3: Identification of PKB-mediated phosphorylation sites on SPEG. | Nature Communications

Fig. 3: Identification of PKB-mediated phosphorylation sites on SPEG.

From: A PKB-SPEG signaling nexus links insulin resistance with diabetic cardiomyopathy by regulating calcium homeostasis

Fig. 3

a–c SPEG fragments were expressed in HEK293 cells that were treated with indicated inhibitors and insulin. PAS-reactive phosphorylation of SPEG was detected on immunoprecipitated SPEG using the PAS antibody. d, e GFP-SPEGP2227-S2583 WT and mutant proteins were expressed in HEK293 cells. PAS-reactive phosphorylation of SPEG was detected on immunoprecipitated proteins using the PAS antibody. f GFP-SPEG WT and mutant proteins were expressed in HEK293 cells. PAS-reactive phosphorylation of SPEG was detected on immunoprecipitated proteins using the PAS antibody. g In vitro phosphorylation of GST-SPEGP2227-S2583 WT and mutant recombinant proteins by PKB. Source data are provided as a Source Data file.

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