Fig. 6: Effects of HFD on calcium homeostasis and phosphorylation of PKB-SPEG-SERCA2a axis in primary cardiomyocytes. | Nature Communications

Fig. 6: Effects of HFD on calcium homeostasis and phosphorylation of PKB-SPEG-SERCA2a axis in primary cardiomyocytes.

From: A PKB-SPEG signaling nexus links insulin resistance with diabetic cardiomyopathy by regulating calcium homeostasis

Fig. 6

a, b. Ejection fraction (EF, a) and fractional shortening (FS, b) in male mice fed with chow diet (CD) or high fat diet (HFD) for 3 months. n = 6 (CD) and 5 (HFD). p = 1.29e-3 (EF) and 7.05e-4 (FS). c Calcium transients in primary cardiomyocytes isolated from male mice fed with CD or HFD upon electrical stimulation. 64 cells from CD-fed mice and 42 cells from HFD-fed mice were analyzed. p = 1.24e-2 (amplitude), 1.79e-7 (FDHM), and 2.52e-4 (Tau). D-E. Phosphorylation of PKB, SPEG and SERCA2a in primary cardiomyocytes isolated from CD- or HFD-fed mice in response to insulin. Phosphorylation of PKB, SPEG and SERCA2a was normalized with their respective total proteins. Represent blots were shown in d, and quantitative data were shown in e. n = 9. The data are given as the mean ± SEM. Statistical analyses were carried out using two-sided t-test for a–c, and two-way ANOVA for e. One-asterisk indicates p < 0.05, two-asterisk indicates p < 0.01, and three-asterisk indicates p < 0.001. One-dagger (CD insulin vs HFD insulin) indicates p < 0.001. Source data are provided as a Source Data file.

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