Fig. 7: Generation and basic characterization of the Speg^3A-knockin mice.

a Diagram of strategy for generation of the Speg^3A-knockin mice. The cluster of serine/threonine residues Ser²⁴⁶¹/Ser²⁴⁶²/Thr²⁴⁶³ (the surrounding sequence is LAVRRRLsstLERL, Ser²⁴⁶¹/Ser²⁴⁶²/Thr²⁴⁶³ shown in lower case, and numbering is according to NP_031489.4) on SPEG was substituted to alanine by point mutagenesis. b Oral glucose tolerance test in the male Speg^3A-knockin mice and wild-type littermates (9-month-old). n = 5 (WT) and 6 (Speg^3A). p = 0.050 (0 min), 0.799 (15 min), 0.996 (30 min), 0.718 (45 min), 0.244 (60 min), and 0.134 (120 min). c Levels of glucose, non-esterified fatty acids (NEFA), triglycerides (TG) and total cholesterol (TC) were determined in the blood of male Speg^3A-knockin mice and wild-type littermates (8-month-old) after an overnight fast. n = 15 (WT) and 11 (Speg^3A). p = 0.270 (blood glucose), 0.317 (plasma NEFA), 0.819 (plasma TG), and 0.412 (plasma TC). d SPEG expression and PAS-reactive phosphorylation in the heart of WT and Speg^3A-knockin mice in response to insulin. The data are given as the mean ± SEM. Statistical analyses were carried out using two-sided t-test. Source data are provided as a Source Data file.