Fig. 2: Omics analysis of ARI-resistant cells reveal altered glucose metabolism.

a Enriched pathways upregulated in the proteomic analysis of ARI-resistant cells when compared to WT LNCaP. Selected proteins were significantly modulated in at least 2 out of 3 conditions. Pathway enrichment analysis was performed using the STRING database (http://string-db.org). b Steady-state levels of significantly regulated metabolites in ARI-resistant cells when compared to WT LNCaP (FC > 1.5, p < 0.05 using a 1-way ANOVA with a Dunnett’s multiple comparisons test). Selected metabolites were significantly modulated in at least 2 out of 3 conditions. c Isotopologue distribution of selected metabolites in ARI-resistant and WT LNCaP cells following ¹³C-glucose incorporation for 1 h. d Isotopologue distribution of pyruvate and phosphoenolpyruvate in ARI-resistant and WT LNCaP cells following ¹³C-glucose incorporation for 24 h. e Isotopologue distribution of reduced glutathione (GSH) in ARI-resistant and WT LNCaP cells following ¹³C-glucose incorporation for 24 h. b–e Data are presented as mean values +/− SD. c–e *p-value of labelled fraction < 0.05 using a 1-way ANOVA with a Dunnett’s multiple comparisons test. DHAP dihydroxyacetone-phosphate, PEP phosphoenolpyruvate, GSH reduced glutathione, GSSG oxidised glutathione. Source data are provided as a Source data File.