Fig. 5: DECR1 is a potential target for CRPC.

a Venn diagrams highlighting proteins commonly modulated (p-value < 0.05, FC > 1.5) in ARI-resistant cells cultured in 2D and 3D conditions. Upregulated proteins are on top; downregulated proteins are into brackets. b Western blot analysis of DECR1 expression in WT and ARI-resistant LNCaP cells. c Western blot analysis of DECR1 expression in AR⁺ prostate cancer cells following acute AR inhibition for 48 h. d Cell proliferation of C4-2 cells silenced for DECR1 expression and treated with enzalutamide (20 µM—48 h). Cell count is normalised to untreated control (siCTL). e Cell proliferation of LNCaP cells overexpressing DECR1 and treated with enzalutamide (20 µM—48 h). Cell count is normalised to untreated empty vector (EV OE). f Western blot analysis of DECR1 expression in hormone naïve (LNCaP, VCaP) and castration-resistant (LNCaP AI, VCaP CR) tumour orthografts. g Immunohistochemical staining (left) and quantification (right) of DECR1 expression in CRPC tissue samples. Data are represented as difference in histoscore between post-treatment and pre-treatment biopsies. Scale bar represents 100 µm. h Percentage of prostate cancer patients showing genomic (copy number gain or amplification) or mRNA alteration (z-score = 1.5) for DECR1 using the TCGA dataset. i Gene expression analysis of DECR1 in normal and tumoural prostate tissues according to the TCGA dataset (n = 498). Centre line corresponds to median of data, top and bottom of box correspond to 75th and 25th percentile, respectively. Whiskers extend to adjacent values (minimum and maximum data points not considered outliers). j Kaplan–Meier survival analysis of prostate cancer patients stratified according to DECR1 expression using the TCGA dataset. b, c, f HSC70 is used as a sample loading control. d, e Data are presented as mean values +/− SD. d, e *p-value < 0.05 using a 1-way ANOVA with a Dunnett’s multiple comparisons test. g statistical analysis was performed using a Wilcoxon matched-pair signed-rank test (n = 14). i statistical analysis was performed using a pairwise ANOVA. j statistical analysis was performed using a logrank test. Source data are provided as a Source data File.