Fig. 2: Par proteins spontaneously form condensed and dynamic puncta in COS7 cells.

a, b Representative images showing subcellular localizations of GFP- or mCherry-tagged Par complex components, including various Par3 fragments (full length, Δ4N12, and Par3N), Par6β, and PKCι, when expressed individually a or mutually b in COS7 cells. Yellow arrowheads indicate condensed puncta in the cytoplasm. Nuclei were stained by DAPI. The lower panel of a is the statistical data for the puncta formation of GPF-tagged Par3 Δ4N12 and Par3N. n = number of independent experimental cell culture batches, with 800 cells counted for each batch. Specimens’ statistics are presented as mean ± SEM; ns, not significant, using one-way ANOVA with Tukey’s multiple comparison test. c Representative time-lapse images showing that the GFP-Par3N and mCherry-Par6β-positive puncta undergo time-dependent fusion. d Representative time-lapse FRAP images showing that GFP-Par3N signal within the GFP-Par3N/Flag-Par6β condensed puncta recovered within a few minutes. e Representative time-lapse FRAP images of GFP-Par6β in the HA-Par3N/GFP-Par6β condensed puncta. f Statistical data for d and e. The red curve represents the averaged FRAP data of 20 puncta from 14 cells. The black curve represents the averaged FRAP data of 25 puncta from 13 cells. Time 0 refers to the time point of the photobleaching pulse. Experiments were performed three times independently with similar results. All data are represented as mean ± SD. All the constructs are listed in Supplementary Table 1. Source data are provided as a Source data file.