Fig. 9: NTD-mediated Par clustering relies on its oligomerization-dependent LLPS behavior.
From: Par complex cluster formation mediated by phase separation
a Representative images of knock-in larval NBs expressing GFP-tagged Baz WT, Baz ΔNTD, or FUSS-Baz chimera visualized with GFP, Par6, aPKC, or Mira. ToPro-3 in blue. Scale bars, 5 μm. b Statistical analysis of ACD protein localization for a. For Baz apical cortical intensity, Baz WT, Baz ΔNTD, and FUSS-Baz, n = 24, 67, and 29 NBs collected from 30 larval brains for each genotype over three independent experiments, respectively. For Par6 apical cortical intensity, w1118, Baz WT, Baz ΔNTD, and FUSS-Baz, n = 10, 10, 26, and 14 NBs collected from 15 larval brains for each genotype over three independent experiments, respectively. For aPKC apical cortical intensity, w1118, Baz WT, Baz ΔNTD, and FUSS-Baz, n = 10, 25, 39, and 32 NBs collected from 15 larval brains for each genotype over three independent experiments, respectively. For Mira basal cortical intensity, w1118, Baz WT, Baz ΔNTD, and FUSS-Baz, n = 10, 34, 46, and 21 NBs collected from 15 larval brains for each genotype over three independent experiments, respectively. c Representative images showing overview of larval brains expressing knock-in GFP-tagged Baz WT, Baz ΔNTD, or FUSS-Baz chimera. d Statistical data measuring brain size presented in for c. n = 10 larval brains over three independent experiments. e, f Representative time-lapse FRAP images showing that recovery of knock-in GFP-Baz WT e or GFP-FUSS-Baz f signal within the preformed crescent occurred within a few minutes. g Statistical data for e and f. For GFP-Baz WT and GFP-FUSS-Baz, n = 13 and 9 NBs collected from 15 larval brains for each genotype over three independent experiments, respectively. h Model for Par proteins local condensation during the ACD of Drosophila NBs. For simplicity, the basal daughter cell was omitted. All the constructs are listed in Supplementary Table 1. For all the statistical data, mean ± 95% confidence interval is shown. ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source data file.
