Fig. 1: Development of md-LED to detect protein–protein interactions.

a The schematic diagram shows the experimental design of md-LED. Human exons library was enriched from fragmented DNA. The DNA fragments were transcribed in vitro and translated. Puromycin was utilized to link mRNA to its encoded protein. The nuclear acid and protein fusion complexes were pre-selected using C-terminus FLAG-tag as an input library. The pre-selected input library was then selected against bait proteins and subjected to high-throughput sequencing to determine the identity and frequency of each exon. b Scatter plot shows the correlation between two independent input libraries. Exon frequencies, shown as reads per million (RPM) were calculated for each replicate and strong correlation were observed with biological duplicate. c The distribution of transcript frequency in input libraries is shown with histogram. Blue bars represent the distribution of exon library, and gray bars correspond to cDNA library.