Fig. 7: Agd3 CBM increases deacetylation rate and specificity. | Nature Communications

Fig. 7: Agd3 CBM increases deacetylation rate and specificity.

From: Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

Fig. 7

a Deacetylation of GAG by Agd3 or point mutants as determined by GAG-ELLA. Experiment performed in triplicate and EC50 calculated from three independent experiments. One representative experiment is data shown as mean values ± one SD. b Time course of α-1,4-(GalNAc)7 deacetylation by Agd3 or Agd3W344A. Products were analyzed by MALDI-TOF MS and graphed as the relative amount of unmodified substrate. The average number of deacetylation events is indicated by the color legend. c Analysis of the MALDI-TOF MS-MS fragmentation of mono-deacetylated heptasaccharide product produced Agd3 or Agd3W344A. The X-axis indicates the location of the deacetylation event within the heptasaccharide. Mean values are of 11 experiments and error bars represent SD. Source data is available in Source Data file.

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