Fig. 5: Design and characterization of multitype NPs clusters assembled with a meshframe of directional and polychromatic valence modes.
a Meshframe programmed with a valence of TBP geometry and encoded ‘colored’ sites at selected vertices, labelled from 1 to 5, results in polychromatic valence. (I–II) two-color valence. (III–V) three-color valence. b Multitype NP clusters. (I) P1(345)P3(12) with three P1 (10 nm AuNP) at sites 3–4–5 and two P3 (20 nm AuNP) on sites 1–2; (II) P1(245)P4(13) with three P1 at sites 2–4–5 and two P4 (30 nm AuNP) at sites 1–3; (III) P1(345) P2(2)P3(1) with three P1 at sites 3–4–5, one P2 (15 nm AuNP) at site 2 and one P3 at site 1; (IV) P1(245) P2(3)P3(1) with three P1 at sites 2-4-5, one P2 at site 3 and one P3 at site 1; (V) P1(24) P2(3)P3(15) with two P1 at sites 2–4, one P2 at site 3 and two P3 at sites 1–5. c Representative negative-stained TEM images of NP hetero-clusters; scale bar, 200 nm (insets: zoomed-in images; side length, 100 nm). d Population histograms for corresponding multitype NP clusters. e Cryo-TEM tomography reconstructed P1(24)P2(3)P3(15) cluster (left). Designed interparticle distances (right, shadow columns): d12 = 69.8 nm, d34 = 58.3 nm, d45 = 60.5 nm, and d35 = 62.6 nm. Measured distances, as averaged over eight independently reconstructed P1(24)P2(3)P3(15) clusters (right, solid columns): d12 = 66.8 ± 4.3 nm, d34 = 61.4 ± 4.6 nm, d45 = 63.8 ± 4.4 nm, and d35 = 67.8 ± 7.2 nm. Error bars indicate standard deviation of experimental data.
