Fig. 1: Postnatal expansion and maturation of RMs parallel renal growth.
From: Distinct fate, dynamics and niches of renal macrophages of bone marrow or embryonic origins
a Representative flow cytometry plots show the percentage of CD11b+F4/80high (cycled area) RMs among CD45 + leukocytes at different ages. b, c Absolute CD11b+F4/80high RMs cell counts (b) and density of RMs (counts/kidney weight) (c) at different ages. Data are pooled from two independent experiments (b: n = 3 mice for P7,14,21; n = 4 mice for P1,105; n = 5 mice for P56. c: n = 3 mice for P1,7,14,21; n = 4 mice for P105; n = 5 mice for P56). d Intracellular staining of Ki67 (proliferative marker) in RMs. Ki67 was detected after surface staining of RMs and the percentage of Ki67+ RMs at different ages is quantified. Data are pooled from two independent experiments (n = 4 mice for P1,91; n = 3 mice for P7,14,21,56). e Representative flow cytometry plots show major histocompatibility II (MHCII) and CD11b expression on RMs over time at P1, 7, 14, 21, and 56. All data are presented as mean ± s.e.m. Source data are provided as a Source Data file.
