Fig. 5: CX3CR1/CX3CL1 axis is required for RMs regeneration under normal and pathologic conditions.
From: Distinct fate, dynamics and niches of renal macrophages of bone marrow or embryonic origins
a Serum CX3CL1 level after ILY injection (120 ng/g b.w.) in Cx3cr1CreER+/− and Cx3cr1CreER+/−/ihCD59+/− mice. Day-0 indicates no ILY injection. Data are pooled from three independent experiments (Cx3cr1CreER+/− group: n = 5 mice for Days 0, 2, and 7 and n = 8 mice for Days 1 and 3; Cx3cr1CreER+/−/ihCD59+/− group: n = 8 mice for Days 0, 1, and 3 and n = 5 mice for Days 2 and 7). b CX3CL1 level in kidney homogenate at 1-day post ILY injection. CX3CL1 protein level was normalized by total protein of homogenate (n = 4 mice per group). c Percentage of RMs (circled area) in CD45+ cells (left, dot plots) and absolute cell counts (right, column graph) at 7 days after ILY injection under CX3CR1 sufficient (+/−) and deficient (−/−) conditions. Data are pooled from two independent experiments. (n = 7 mice per group). d Schematic overview of the experiment. CD45.1+ WT male mice were lethally irradiated and reconstituted with WT, Cx3cr1−/− or Ccr2−/− BM (CD45.2+). Regeneration of tissue macrophages (mac) or monocytes in kidney, spleen, and lung was determined 5 weeks later. e, f Number of donor-derived TMs and monocytes in kidney, spleen, and lung 5 weeks after BMT. e WT vs Cx3cr1−/− (f) WT vs Ccr2−/− (n = 4 mice per group). All data are presented as mean ± s.e.m. p-values by two-tailed unpaired t-test are indicated in a–c, e, f. Source data are provided as a Source Data file.
