Fig. 1: Single-cell transcriptome profiling of OHBBN-induced mouse bladder tumors.
From: Epithelial plasticity can generate multi-lineage phenotypes in human and murine bladder cancers
a tSNE plots showing a total of 22 clusters identified from CD45-negative and CD45-positive fractions isolated by flow cytometry from OHBBN-induced primary bladder tumors (pooled tumors, n = 3 tumors). b tSNE plots showing the proximity of cells with high expression of genes belonging to basal, luminal, and EMT-claudin lineage subtypes within Epcam-positive defined populations. c Single-cell gene plots gated on cells with high Krt5 and Krt8 gene expression followed by the assessment of EMT-claudin genes (Cldn4, Cldn7). Cells with Krt5 > 0 and Krt8 > 0 expression were gated as Krt5-Krt8 high, whereas cells with Krt5 = 0 and Krt8 = 0 were gated as Krt5-Krt8 low. Axis units are log (UMI) or transformed transcripts per cell. d tSNE plots showing the presence of bi-lineage-positive cells using gene expression overlays from (left) basal + luminal, (middle) luminal + EMT-claudin, and (right) basal + EMT-claudin subpopulations. Tumor identification and cell numbers sequenced were as follows: tumor 4950 (CD45-neg = 2939 cells, CD45-pos = 1307 cells), tumor 8524 (CD45-neg = 6119 cells, CD45-pos = 2736 cells), and tumor 8525 (CD45-neg = 5068 cells, CD45-pos = 7564 cells). Genes assessed in tSNE plots are shown in Table 1. Top up and down genes are shown in Supplementary Data 1.
