Fig. 2: Detection of OHBBN-induced bladder cancer cells with single-, double-, and triple-lineage marker-positive cells.
From: Epithelial plasticity can generate multi-lineage phenotypes in human and murine bladder cancers
a Single-cell RNA-seq analysis showing the presence of epithelial cells high in basal (Krt5), luminal (Krt8), and EMT-claudin (Cldn3, Cldn4, and Cldn7) marker gene expression. b Low-magnification triple immunofluorescence detection of Ck5 (Krt5), Ck8 (Krt8), and Cldn7 (Cldn7), and the corresponding hemotoxylin & eosin (re-stain of IF slide). c High-magnification views of insets from b showing instances of tumor cells co-expressing Ck5-Cldn7-Ck8low (solid arrows), Ck8hi-Cldn7 (open arrows), and Ck5-Cldn7-Ck8 (open dashed arrows). Each experiment was performed at least in triplicate.
