Fig. 3: Tumor endothelial cells and myofibroblasts promoting angiogenesis and tissue remodeling. | Nature Communications

Fig. 3: Tumor endothelial cells and myofibroblasts promoting angiogenesis and tissue remodeling.

From: Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma

Fig. 3: Tumor endothelial cells and myofibroblasts promoting angiogenesis and tissue remodeling.The alternative text for this image may have been generated using AI.

a tSNE plot of endothelial cells, color-coded by clusters and cell subsets as indicated. EPCs: endothelial progenitor cells. b Three-layered complex heatmap of selected endothelial cell marker genes in each cell cluster. Top: Mean expression of known lineage markers. Middle: Tissue preference of each cluster; Bottom: Relative expression map of known marker genes associated with each cell subset. Mean expression values are scaled by mean-centering, and transformed to a scale from -2 to 2. c Average cell number and relative proportion of EC subsets from tissues of each origin. nLung, n = 11 samples; tLung, n = 11 samples; tL/B, n = 3 samples; mLN, n = 2 samples; mBrain, n = 9 samples. d Functional association networks between signature genes specific to tumor ECs. e tSNE plot of fibroblasts (FBs), color-coded by clusters and cell subsets as indicated. f Average cell number and relative proportion of fibroblast subsets from tissues of each origin (excluding undetermined cells). nLung, n = 11 samples; tLung, n = 11 samples; tL/B, n = 3 samples; mLN, n = 4 samples; mBrain, n = 10 samples. g Complex heatmap of selected fibroblast marker genes in each cell cluster, as shown in (b) (excluding undetermined cells). h, IHC staining of α-SMA on formalin-fixed and paraffin-embedded slides for the independent biospecimens (n = 4). All replicates showed the similar results. i, Representative flow cytometry plots showing myofibroblast (α-SMA+) in primary tumor (T06) and normal lung (N06) tissues. j, Box plot of the percentage of myofibroblast (α-SMA+) in normal and tumor-derived EPCAM−CD45− cells. nLung (n = 5; N06, N18, N41, N42, N43) and tLung (n = 5; T06, T18, T41, T42, T43). SMC and pericytes can be included in the α-SMA + cells as minor populations. *p < 0.05 (p = 0.02), two-sided Student’s t test. Each box represents the interquartile range (IQR, the range between the 25th and 75th percentile) with the mid-point of the data, whiskers indicate the upper and lower value within 1.5 times the IQR.

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