Fig. 2: Activation and recovery kinetics of optogenetic sequestration systems.
a, b Fluorescence micrographs of mCherry-labeled bait proteins in the iLID-based (a) and LOV-based (b) sequestration systems, before (left) and directly after (right) illumination with blue light. Size bar, 2 µm; insets 2× enlarged. c, d Representative fluorescence signal quantification across bacteria over time in the iLID-based (c) and LOV-based (d) sequestration systems; dark gray: membrane, light gray: cytosol. Insets: Fluorescence relocalization factor (fluor. reloc. = Rpost-light/Rpre-light, where R represents the ratio of fluorescence intensities at the membrane and in the cytosol, before and after illumination, respectively), based on 121−131 line scans across five cells per strain and time point. Single relocalization factor values (n = 5) indicated by circles. Bars show mean values; error bars represent the standard deviation, *p = 0.0002; ***p = 6.2 × 10−6 against no relocalization in a two-tailed homoscedastic t test. Source data for panels (c) and (d) are provided as a Source Data File.
