Fig. 6: Heterologous cargo can be exported in a light-dependent manner. | Nature Communications

Fig. 6: Heterologous cargo can be exported in a light-dependent manner.

From: LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution

Fig. 6

a In vitro secretion assay showing light-dependent export of YopE1-53-NanoLuc-FLAG (see scheme below; exp. size, 28.7 kDa), in the indicated strains. Western blot using anti-FLAG antibodies for the culture supernatant of 3 × 108 bacteria per strain. Left side, molecular weight in kDa. b Quantification of light-dependent YopE1-53-NanoLuc-FLAG export by densitometric analysis of Western blots, normalized by average secretion of the wild-type control (WT), N = 3 densitometry values from independent experiments. Single data points indicated by circles, error bars display the standard error of the mean. L/D ratio, ratio of secretion under light and dark conditions. Source data are provided as a Source Data File.

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