Fig. 2: Universally conserved residues of TBP-lobe like regions and their functional relevance. | Nature Communications

Fig. 2: Universally conserved residues of TBP-lobe like regions and their functional relevance.

From: Molecular determinants underlying functional innovations of TBP and their impact on transcription initiation

Fig. 2

a The spatio-temporal context denotes a phylogenetic range of all the three superkingdoms of life (temporal) and the consideration of entire TBP lobe-like regions (spatial). NA-binding residues are highlighted with ‘*’. CTN position L5.1 contains an almost universally conserved Phe residue in eukaryotes and archaea. Residues at L5.1 are shown in ball and stick representation, and highlighted with black circles on the structures (right). b Universally conserved positions are moderately preserved in TBP lobe-like sequences of dsDNA viruses and the most prominent L5.1 position is a Phe in most viruses. This suggests TATA-box sequence binding potential for viral TBP-like sequences. c In bacteria, L5.1 position predominantly contains a conserved Tyr or a His as opposed to Phe at this position in archaea, eukaryotes, and viruses. The structures of TBP lobe-like regions show that the aromatic residues of L5.1 are oriented in a similar manner across the three superkingdoms. The PDB identifiers for structures are 1cdw, 4py5, and 4i8o from top to bottom.

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