Fig. 1: Single-cell analysis of murine postnatal heart development. | Nature Communications

Fig. 1: Single-cell analysis of murine postnatal heart development.

From: Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation

Fig. 1

a, b t-distributed stochastic neighbor embedding (t-SNE) clustering of single cells from postnatal day 1 (P1), P4, P7, P14, and P56 hearts. CCA was applied to remove batch effects. Cells were colored by cell type (a) or time point (b). CM cardiomyocyte, EC endothelial cell, FB fibroblast, MP macrophage, SMC smooth muscle cell, TC T cell, GR granulocyte. c Cell populations in a were identified by the expression of established marker genes. d, e Monocle analyses showing the ordering of CMs in pseudotime. Each color indicates either a time point (d) or a cell state (e). f State proportions of CMs from P14 or P56 hearts, respectively. g–j Gene ontology (GO) analysis of genes specifically expressed in a given CM state from e. Selected top categories are shown here (Supplementary Data 1–6). k Heatmap to display different blocks of top 1000 differentially expressed genes (DEGs) along the pseudotime trajectory (d, e). Please see Supplementary Data 7 for the full list. Right: selected top Kyoto Encyclopedia of Genes and Genomes (KEGG) terms related to corresponding DEGs in blocks significantly changed during cardiomyocyte maturation (P1–P56), Functional analysis was performed with enrichKEGG in clusterProfiler, p < 0.05 was considered significant enrichment.

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