Fig. 7: NELF potentiates inflammatory responses secondary to constraining the production of IL-10.

a ELISA for IL-6 (n = 5, P = 0.0051), TNF (n = 5, P = 0.0402) and IL-12p40 (n = 4, P = 0.0061) protein levels in WT and Nelfb KO BMDM stimulated with LPS for 6 h. *P < 0.05, **P < 0.01 by two-sided paired Student’s t test. b WT and Nelfb KO BMDM pretreated with 2 μg/ml of anti-IL10R for 0.5 h, where indicated, were activated with LPS for 6 h. IL-6 levels in culture supernatants were measured by ELISA and shown as relative values normalized to levels in WT cells that were set as 1 in each independent experiment (n = 3). c FACS analysis of the macrophage population (CD45⁺CD11b⁺F4/80⁺) in peritoneal exudates of WT and Nelfb KO mice 9 h after intraperitoneal injection of LPS (100 ng/mouse). Representative FACS distribution (left) and cumulative (n = 4) percentage (middle, P = 0.0002) as well as absolute numbers (right, P = 0.0014) are shown. d Cumulative data showing total peritoneal cell numbers from WT and Nelfb KO mice 9 h after LPS injection (n = 7, P = 0.8232). e RT-qPCR analysis of Il6 in peritoneal cells of WT and Nelfb KO mice 9 h after intraperitoneal injection of LPS (n = 6, P = 0.0074). **P < 0.01 by two-sided paired Student’s t test. f FACS analysis of macrophage population (CD45⁺CD11b⁺F4/80⁺) in peritoneal exudates of WT and Nelfb KO mice 9 h after intraperitoneal injection of LPS or LPS + IL-6 (40 ng/mouse). Representative FACS distribution (left) and percentage of macrophages from independent experiments (n = 4–6) (right, WT + LPS/Nelfb KO + LPS P = 0.0008; Nelfb KO + LPS/Nelfb KO + LPS + IL-6 P = 0.0105) are shown. For c, d, and f, *P < 0.05, **P < 0.01, ***P < 0.001, N.S. P > 0.05 by two-sided unpaired Student’s t test. n represents biologically independent animals. Error bars indicate SEM. Source data are provided as a Source Data file.