Fig. 1: Proposed mechanisms of MavC-catalyzed reactions and constructs used for structural studies.
From: Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination
a Proposed mechanism of ubiquitination resulting from transglutamination reaction catalyzed by MavC. Thioester formed in Step 1 may undergo either attack by the amino group of Ube2N Lys92 (leading to formation of Ub–Ube2N) or hydrolysis (formation of deamidated Ub). Key residues from MavC in burgundy, Ube2N in light green, and Ub in teal are represented. b Diagram depicting protein constructs used for crystallization studies and NMR experiments and location of the MavC insertion domain (residues 128–225), with diagrams of Ube2N–Ub, Ube2N-SS-Ub (MavC substrates), and Ub–Ube2N (MavC product) provided for comparison.
