Fig. 4: Binding interface of MavC with Ub.
From: Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination
a, b Views of the MavC•Ub-binding interface showing interactions between residues of MavC (burgundy) and Ub (teal). Hydrogen-bonding interactions are shown in black dashed line and hydrophobic contacts in red dashed line. c Native-PAGE gel comparing Ub deamidation activities of Ub-binding mutants of MavC. Reactions were visualized with Coomassie Blue. A control reaction without MavC is included. d Comparison of the ubiquitinating activity of the Ub-binding mutants of MavC using Ube2N-SS-Ub as the substrate. Reactions were subjected to SDS-PAGE and visualized with Coomassie Blue. A control reaction without MavC is included. e Architecture of the active site in the structure of MavC substrate complex (P65), with Ala74 of MavC modeled as a Cys. Interactions holding Gln40Ub in position are described.
