Fig. 2: Splicing machinery bound to Pir2 mediate gene repression by lncRNA.
From: Conserved protein Pir2ARS2 mediates gene repression through cryptic introns in lncRNAs
a Co-IP analysis of Pir2-GFP with Cwf10-FLAG proteins. Source data are provided as a Source data file. b Glycerol gradient analysis. Elution fractions are indicated above the gel. Source data are provided as a Source data file. c Northern blot analysis of transcripts produced from the pho1 and byr2 loci. Probes are described in Fig. 1a. Source data are provided as a Source data file. Cells were grown in YEA medium. d RT-qPCR analysis of pho1 and byr2 expression, normalized to leu1. The amplified regions are indicated by the black lines. Data are presented as mean values ± SD for n = 3 biologically independent samples. Mean data distribution is represented by white circles. e Representative DIC images of homothallic (h90) WT and mutant strains. The sporulation frequency is indicated as a percentage (n > 1000). Insets show colonies stained with iodine vapor. Indicated white scale bar represents 10 µm.
