Fig. 6: UDM1 is required for RNF168-mediated 53BP1 recruitment.
From: Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination
a Schematic illustration of RNF8 and RNF168 structural domain organization, RNF168 fragments, and chimeric proteins used as in b and c. FHA–forkhead-association domain; coiled-coil–coiled-coil domain; RING–ubiquitin E3 ligase RING domain; Rs-arginine anchor; LRM–LR motif; UMI–UIM-and MIU-related ubiquitin binding domain; MIU–motif interacting with ubiquitin. b–c Transient transfection of GFP-tagged RNF168 fragments and chimeric proteins in U2OS RNF168 KO cells. Cells were irradiated with 10 Gy and allowed to recover for 1 h followed by immunofluorescence analysis using 53BP1 antibody as indicated. Cells were counterstained with DAPI. Repeated at least two times independently with similar results. Source data are provided as Source Data file.
