Fig. 2: Knocking down a mitochondria-associated protein, Dosmit, results in significant differences in mitochondrial size and shape. | Nature Communications

Fig. 2: Knocking down a mitochondria-associated protein, Dosmit, results in significant differences in mitochondrial size and shape.

From: Vesicular transport mediates the uptake of cytoplasmic proteins into mitochondria in Drosophila melanogaster

Fig. 2: Knocking down a mitochondria-associated protein, Dosmit, results in significant differences in mitochondrial size and shape.The alternative text for this image may have been generated using AI.

a–d Western blots highlighting expression levels of mitochondrial proteins: a Marf, b Opa1, c Drp1, and d Fis1. e Confocal microscopy of mitochondria in control and Dosmit-knockdown (KD) flies, highlighting the size and shape of mitochondria. Scale bar: 10 μm. f Mitochondrial size in control and Dosmit-knockdown flies (mean ± SD). N = 126 and 144 from left to right bars. Statistical test: Two-tailed Mann–Whitney U-test (****p < 0.0001). g Immunofluorescent staining of muscles of wild-type flies: ATP5A (green) in the inner membrane of mitochondria; F-actin (blue) in muscle fibers, between which oval-shaped mitochondria are aligned; and Dosmit (red) localized to mitochondria. Scale bar: 10 μm. h Western blotting of flight-muscle tissue homogenates indicating the presence of Dosmit protein in the mitochondrial fraction only (M, mitochondrial fraction; C, cytosolic fraction; tubulin: cytosolic marker; Dosmit*: Dosmit dimer). i Western blotting of flight-muscle testing the presence/absence of Dosmit protein in tissue homogenates from control and homozygous Dosmit-EP mutant flies (GAPDH: loading control). j Confocal microscopy of mitochondria in the flight muscles of control and homozygous Dosmit-EP flies. Color panels are confocal microscopy images; gray-scale panels are TEM images. Scale bar of fluorescence images: 10 μm; scale bar of TEM images: 1 μm. k Size (mean ± SD) of mitochondria in flight-muscle of wild-type and Dosmit-EP flies. N = 91 and 131 from left to right bars. Statistical test: Two-tailed Mann–Whitney U-test (****p < 0.0001). Source data are provided as a Source Data file.

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