Fig. 4: Increased survival to cold exposure correlates with LD accumulation during aging.
From: Independent regulation of age associated fat accumulation and longevity
a–c Cells as indicated were aged in 30 °C media until median age 16 (middle-age), and then exposed to 4 °C (blue lines) or 30 °C (black lines) media at regular intervals until death (see Methods), and lifespan was analyzed as in Fig. 1f (see Methods). Log-rank test: p > 0.05, not significant; ****p ≤ 0.0001. a Control (WT, AB18-07, 550 cells at 30 °C, and 525 cells at 4 °C), b BNA2-OE aro1Δ (525 cells at 30 and 4 o C), and c BNA2-OE (550 cells at 30 and 4 °C) were analyzed over n = 4 independent experiments. Average median and maximal lifespans at 30 °C (±SEM): Control 25.8 ± 1.3, 41 ± 1.9; BNA2-OE aro1Δ 25.1 ± 2.2, 44.3 ± 4.2; BNA2-OE 29.5 ± 1.7, 48.8 ± 2.5. Average median and maximal lifespans at 4 °C (+SEM): Control 27.5 ± 1.3, 43.3 ± 2.5; BNA2-OE aro1Δ 25 ± 1.8, 45 ± 4.6; and BNA2-OE 24.8 ± 1.3, 40 ± 3.7. Source data for (a–c) are provided as a Source data file.
