Fig. 4: Transcriptional noise and bursting of the σ70 controlled Suf system.
From: The route to transcription initiation determines the mode of transcriptional bursting in E. coli
Wildtype, Δfur and ΔoxyR E. coli cells were grown in absence (no stress) and in presence of either H2O2, BP or BP+H2O2. The cells were then subjected to RNA fluorescence in situ hybridization with probes against sufABCD mRNA30. a Noise (CV2 = σ2/μ2) and burstiness (Fano factor F = σ2/μ) of sufABCD transcription under each condition (top) and as a function of the mean sufABCD mRNAs per cell (bottom). CV2 and F were calculated from the mean and standard deviations associated with the mRNA distributions in Supplementary Fig. 6. Data are presented as the statistic of the full set of data samples +/− the SEM obtained from n = 10,000 bootstrap resamples. b Burst kinetics of sufABCD transcription under each condition (top) and as a function of the mean sufABCD mRNAs per cell (bottom). Data are presented as the maximum a posteriori estimate (measure of centre of the error bar) and error bars are 95% credible intervals. Both are derived from the MCMC posterior distributions.
