Fig. 2: Protection from intestinal ischemia/reperfusion injury due to Tipe0 gene deletion is immune-cell independent and occurs during the ischemic phase of injury in contrast to Tipe2 gene deletion.

a, b Histology from bone marrow chimeras subjected to I/R90′, along with adjacent healthy tissue controls. Bars = 220 µm, inserts are 4× magnified, with blinded histology scores (b); Number of mice/group as follows: WT- > WT healthy = 4, WT- > Tipe0^−/− healthy = 8, WT- > Tipe2^−/− healthy = 3, WT- > WT ischemia = 5, WT- > Tipe0^−/− ischemia = 6, WT- > Tipe2^−/− ischemia = 6; images represent the mean histological score of each group. c Blinded histology scores and d representative images of mice subjected to 60 min of ischemia with no reperfusion time (I60′). N = 5 mice/group except WT Healthy and Tipe0^−/− I/R60′, where N = 6; images represent the mean histological score, bars = 220 µm, inserts are 4× magnified. e, f CD11b staining (green) of WT and Tipe2^−/− mice subjected to I60′ and I/R90′, image representative of 4/4 mice/group; bars = 100 µm; quantification in f. For all panels: p as indicated in the figure. For all graphs, error bars are mean ± SEM. Multiple group comparisons were by Kruskal–Wallis one-way ANOVA. Two-tailed Mann–Whitney U test was used to confirm ANOVA findings. Source data are provided as a source data file.