Fig. 7: Nuclear YAP localization is inhibited in budding Tipe0^−/− enteroids while Clu is induced in Tipe0^−/− colons.

a RT-PCR assay for Tnfaip8 (variant 1) gene expression. N = 8 for WT ileum, five for radiation, and four for all other groups; p = 0.0016 for radiation, p = 0.0286 for DSS. YAP staining in 2-day enteroids, bars = 20 µm; insert is 3× magnified, with quantification of nuclear YAP by ICQ in developing crypts (c). N = 10 for WT, N = 12 for Tipe0^−/−, pooled from two independent experiments, with images representative of those quantified; p = 0.0016. d, e RNAscope Clu⁺ cells in mice WT and Tipe0^−/− mice, before and after DSS treatment with additional staining for Ki-67, Clu, and Lgr5, with DAPI counterstain. Ki-67 and Lgr5 are used as controls to identify enterocyte specific staining for further quantification (d). For healthy tissues, N = 9/group (3 mice/group, with 3 images/group analyzed). For DSS tissues, N = 20 (five images each from N = 4 mice/group were analyzed). Images are representative of those quantified; Bars = 40 µm; insert is 5× magnified. e Data analyzed one-way ANOVA with Sidak′s multiple comparison test. For all graphs, data analyzed by two-tailed Mann–Whitney U test unless otherwise specified; *p < 0.05; **p < 0.01 vs WT; error bars show mean ± SEM. Source data are provided as a source data file.