Fig. 3: GEE disrupts in vivo habitual action encoding in DLS circuits.

a Schematic diagram showing site of multi-electrode recording in the DLS (black) and DMS (gray), and experimental design. In vivo recordings were made on day 1 and 6 of schedule training and outcome devaluation (DV) testing (marked in red). (b) Response rate during RI and RR schedule training for CE and GEE mice that were implanted with multi-electrode arrays. c, e Representative raster plots (top-panel) and peri-event time histograms (bottom-panel) of putative DLS MSNs increasing (left) or decreasing (right) their activity around the lever-press (red line at time 0 (s)) in CE (c) and GEE (e) mice. Each row in the raster is neural activity +5 to −2 s around a lever press (time = 0 s, red line). Trials are sorted according to the order of lever-presses made across the session. The peri-event time histogram shows the average firing rate during lever-press related behavior. d Normalized lever-pressing in RI and RR trained contexts during outcome revaluation testing in Valued (V) and Devalued (DV) states in CE and GEE mice implanted with multi-electrode arrays. f Baseline firing rate of putative DLS MSNs that fired in both RI and RR training contexts in CE (n = 239) and GEE (n = 281) mice. g Proportion of putative DLS MSNs per animal that changed firing rate during lever-pressing under RI and RR schedules during training (day 1 and day 6) and following outcome devaluation. h, i The % rate net modulation during lever-press related behavior of firing rate in CE and GEE mice in h RI and i RR contexts. Inset shows percentage rate modulation distribution of individual units on Days 1, 6, and DV. Error bars equal ± SEM, * = p < 0.05.