Fig. 7: GEE alters tonic endocannabinoid modulation of GABAergic transmission that can be partially rescued by decreasing the endocannabinoid tone.

a Representative traces of MSNs recorded from CE and GEE before (baseline) and during exposure to WIN55-212,2, a CB1R agonist. b Graph showing WIN55-212,2 effects on frequency of mIPSCs in CE (black) and GEE (red) mouse MSNs. Note the loss of agonist effect in GEE mouse neurons. c Representative traces of MSNs recorded from CE and GEE before (baseline) and during exposure to AM251, a CB1R antagonist. d AM251 increases the frequency of mIPSCs in GEE but not CE. e Representative traces of MSNs recorded from CE and GEE before (baseline) and during exposure to tetrahydrolipstatin (THL), a DAGL inhibitor. f Graph showing THL effects on mIPSCs frequency in CE and GEE mice, with increases only observed in GEE neurons. g Representative traces of MSNs recorded from CE and GEE before (black trace), after a 4-s membrane depolarization (DSI) (red trace) and ~8 min after DSI (blue tracE). h Graph showing DSI effects on mIPSC frequency in CE and GEE mice. The black, red and blue arrows denote the time at which the corresponding colored traces in g were taken. Error bars equal ± SEM, * = Repeated measures ANOVA Bonferroni corrected p < 0.05.