Table 1 Statistics of cryo-EM data collection and refinement.

From: Parkinson’s disease associated mutation E46K of α-synuclein triggers the formation of a distinct fibril structure

NAME

Ac-E46K α-syn fibril

PDB ID

6L4S

EMDB ID

EMD-0833

DATA COLLECTION

Magnification

105,000

Pixel size (Å)

0.665

Defocus Range (μm)

−1.5 to −2.4

Voltage (kV)

300

Camera

K2 summit

Microscope

Titan Krios

Exposure time (s per frame)

0.25

Number of frames

32

Total dose (e- per Å2)

50

RECONSTRUCTION

Micrographs

754

Manually picked fibrils

13,064

Box size (pixel)

160

Inter-box distance (Å)

19

Segments extracted

806,640

Segments after Class2D

49,902

Segments after Class3D

18,009

Resolution (Å)

3.37

Map sharpening B-factor (Å2)

−97.805

Helical rise (Å)

2.38

Helical twist (°)

−179.37

ATOMIC MODEL

Initial model used (PDB ID)

2N0A

Map sharpening B-factor (Å)

−90.924

Non-hydrogen atoms

2,268

Protein residues

330

Ligands

0

r.m.s.d. bond lengths

0.014

r.m.s.d. bond angles

1.359

All-atom clashscore

7.71

Rotamer outliers

0.00%

Ramachandran Butliers

0.00%

Ramachandran Allowed

5.66%

Ramachandran Favored

94.34%

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