Fig. 2: The FACT complex represses initiation of intragenic isoforms. | Nature Communications

Fig. 2: The FACT complex represses initiation of intragenic isoforms.

From: Transcript isoform sequencing reveals widespread promoter-proximal transcriptional termination in Arabidopsis

Fig. 2

a Pie chart of TIF-cluster categories proportions present in spt16-1. b Pie chart of TIF-cluster categories proportions present in ssrp1-2. c, d Genome browser screenshots of the genes AT5G51200 (c) and AT4G15260 (d). TSS-seq data (top) for wild type, spt16-1 and ssrp1-2, with fact-repressed intragenic TSS in red. TSS/PAS pairs by TIF-seq (below) are indicated as lines connecting TSSs (blue) and PASs (orange). Inverted TIF-cluster orientation for spt16-1 in d indicated antisense lncRNA isoforms. e Schematic representation of illustrating profile of TIF-TSS/PAS pairs representing internal initiation. TIF-cluster and 2D illustration of their positions with respect to genome annotations are indicated in green lines and circles. f Scatterplot representing the TSS/PAS pair distance to annotated TSS/PAS for a subset of TIF-clusters from the combined spt16-1 and ssrp1-2 TIF-seq data sets. The selected data strictly overlap the published fact-specific TSSs genomic positions identified through TSS-seq differential expression analysis in both spt16-1 and ssrp1-2 mutants. The colour scale represents 2D position enrichment density of clusters from low (grey) to high (yellow). Source data of a, b are provided in the Source Data file.

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