Fig. 6: Autophagosomes and autophagic flux increase in the absence of ISG15/ISGylation.
From: ISG15 and ISGylation is required for pancreatic cancer stem cell mitophagy and metabolic plasticity
a Transmission electron micrographs of control and ISG15CRISPR Panc185 cells. Scale bars = 5 or 2 µM. b Immunofluorescent images of LC3B (red) and LAMP1 (green) in control and ISG15CRISPR Panc185 cells. Nuclei stained with DAPI in blue. Scale bar = 10 µM. c WB analysis of LC3B in control and ISG15CRISPR cultures untreated (Ctl) or treated with the autophagy inhibitor Bafilomycin (Baf) for 5 h. Tubulin was used as loading control. d Mean fold change ± sd in autophagic flux determined by densitometric quantification of LC3B-II accumulation after autophagy inhibitor addition, subtracting their respective control expression levels (Panc185: n = 4 independent experiments; *p = 0.0162, as determined by Student’s t-test; and Panc354: n = 3 independent experiments; *p = 0.0143, as determined by Student’s t-test).
