Fig. 5: MYC transcriptionally represses the STAT1/2-Type I IFN signaling associated with NK cell maturation. | Nature Communications

Fig. 5: MYC transcriptionally represses the STAT1/2-Type I IFN signaling associated with NK cell maturation.

From: MYC functions as a switch for natural killer cell-mediated immune surveillance of lymphoid malignancies

Fig. 5

a Quantitative real-time PCR for MYC in P493-6 BL human cells before and after MYC inhibition for 24 h by doxycycline (n = 3, mean ± s.d.). b Phospho mass cytometry (Phospho-CyTOF) depicting changes in pSTAT1 levels before (MYCON) and after (MYCOFF) MYC inactivation for 24 h in P493-6 cells. c Immunoblotting to validate changes in pSTAT1 observed by phospho-CyTOF. d Heatmap depicting transcriptional changes in STAT1/2-Type I IFN signaling cascade before (MYCON, n = 6) and after MYC inactivation (MYCOFF, 24 h, n = 4) in P493-6 cells using expression arrays. e Quantitative real-time PCR for Type I IFNα2 in P493-6 BL human cells before and after MYC inactivation for 24 h by doxycycline (n = 3, mean ± s.d.). f Luminex assay depicting changes in Type I IFNα2 secreted from P493-6 cells before and after MYC inactivation for 24 and 48 h. Each time point was carried out in duplicates. Average of these duplicates is shown. g Immunoblotting in P493-6 BL cell lines expressing empty vector (EV), MYC overexpression vector (MYC), or a MYC mutant that fails to bind MIZ1 (MYC V394D), pre- (Tet-MYCON) and post (Tet-MYCOFF) MYC inactivation. h–k Quantitative real-time PCR for MYC (h), STAT1 (i), STAT2 (j) and Type I IFNα2 (k) in P493-6 BL cell lines expressing empty vector (EV), MYC overexpression vector (MYC), or a MYC mutant that fails to bind MIZ1 (MYC V394D), pre- (Tet-MYCON) and post (Tet-MYCOFF) MYC inactivation (n = 3, mean ± s.d.). All p-values have been calculated using two-tailed Student’s t-test. ns not significant.

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