Fig. 2: Translatome (protein output) remodeling by hypoxia-adaptive RBPs.
From: A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism
a Schematic of TMT-pSILAC translatome analysis strategy. Color scheme: red, normoxia; blue, hypoxia. b Hypoxic translatome (protein output) remodeling. Class III: hypoxia-enriched proteins (at least 15% increase protein output under hypoxic conditions); Class II: oxygen-neutral proteins (no change in protein output); Class I: normoxia-enriched proteins (at least 15% decrease in protein output under hypoxic conditions). c Classical HIF/transcriptionally induced targets represent ~10% of all Class III proteins. Left panel: of these, 86% are regulated by the top five hypoxia-adaptive RBPs identified by MATRIX. Right panel: percentage and number of proteins regulated by each RBP. d Left panel: the majority (72%) of all Class III, hypoxia-inducible proteins are regulated by the top five hypoxia-adaptive RBPs identified by MATRIX. Right panel: percentage and number of proteins regulated by each RBP. e Representative immunoblots of U87MG treated with siRNAs (for 48 h prior to following experimentation) against hypoxia-adaptive RBPs. NS: non-silencing. Quantitation represents mean of three independent experiments (n = 3). f Classification of proteins downregulated at the production level by knockdowns of indicated RBPs. p values represent Chi-Square tests for significant proportional differences compared to overall hypoxic translatome remodeling (presented in b). g Representative immunoblots of U87MG treated with siRNAs against one or two hypoxia-adaptive RBPs. NS: non-silencing. Quantitation represents mean of three independent experiments (n = 3). Source data are provided as a Source data file.
