Fig. 4: Computational prediction of a circadian kinome.

a The distribution of protein kinase groups predicted to phosphorylate NCPs. The top five groups of kinases are CMGC (cyclin-dependent kinase [CDK], mitogen-activated protein kinase [MAPK], glycogen synthase kinase [GSK3], CDC-like kinase [CLK]), AGC (protein kinase A, G, and C), CAMK (calcium/calmodulin-dependent protein kinase), STE (Ste20 kinase), and CK1 (casein kinase I). b The top 10 kinase families predicted to phosphorylate NCPs. c Each one of the four parts of the circle includes all 169 kinases that can be predicted by GPS 2.1. Each bar indicates a kinase. Kinases that oscillate at mRNA, protein or phosphorylation level are indicated in orange. Predicted circadian kinases are also indicated in orange. Among the kinases labeled in orange, the same kinase is connected by a line. d 27 potential circadian kinases predicted by iCMod, including seven that are already known to regulate the clock (red) and 20 that potentially participate in circadian regulation (green). Predicted circadian kinases that oscillate at mRNA, protein, or phosphorylation level are indicated. e Seven kinases previously known to regulate the clock are labeled in blue. Three kinases are found to be involved in modulating locomotor rhythm (labeled in red), and three more are found to be potentially involved (labeled in orange). The remaining 14 predicted circadian kinases are labeled in gray.