Fig. 1: Huh-106 are less permissive to HBV infection than HepG2-NTCP.
From: A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor
a HBV and HDV infection of HepG2-NTCP and Huh-106 cells and detection of HBsAg and HDAg by IF after 10 dpi. One representative experiment is shown. Scale bars: 100 µm. b Binding of HBV particles to HepG2-NTCP and Huh-106 cells. Results are expressed as means +/− SEM bound HBV genome copies (%) from three independent experiments (n = 8). c Comparison of HBV cccDNA levels in HepG2-NTCP and Huh-106 cells detected by Southern blot. Protein-free relaxed circular DNA (pf-rcDNA), double stranded linear DNA (dsl DNA), and covalently closed circular DNA (cccDNA) are indicated. One representative experiment is shown. d Quantification of cccDNA band intensity. Dashed line indicates the detection limit (DL). Results are expressed as means +/− SEM 106 band intensity (arbitrary units) from 4 independent experiments. e Time course experiment of HBV infection in Huh-106 and HepG2-NTCP. DNA was extracted from cells 2 (D2), 4 (D4), or 9 (Mock, D9) days post HBV infection and detected by Southern blot. Bands of pf-rcDNA, dsl DNA, and cccDNA were identified using a molecular marker (MM). One experiment is out of three shown. Quantification of cccDNA band intensities in Fig. S5a. f–h Quantification of intracellular pgRNA by qRT-PCR (f) and secreted HBsAg (g) and HBeAg (h) by CLIA in Huh-106 and HepG2-NTCP cells 1 (D1), 4 (D4), 7 (D7), or 10 (Mock, D10) days post HBV infection. f Results are expressed as means +/− SEM relative pgRNA expression from four experiments (n = 13). g Results are expressed as means +/− SEM IU/mL HBsAg from 4 experiments (n = 12). h Results are expressed as means +/− SEM PEI U/mL HBeAg from 4 experiments (n = 12). MM molecular marker. Source data are provided as a Source Data file.
