Fig. 2: Gain-of-function (GOF) screen in Huh-106 cells for the identification of HBV host factors.
From: A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor
a Schematic workflow of GOF screen. b FACS for HBsAg-positive cells in Huh-106 transduced with an ORF library (hORFeome v8.1) 10 days after HBV infection (HBV pre-sort). Flow cytometric analysis of uninfected cells as gating control (Mock) and of the HBsAg-positive sorted population as sorting control (HBV sorted). c, d Primary screen candidates. ORFs with Log2FC > 1.5 were selected for validation. Source data are provided as a Source Data file.
