Fig. 4: CDKN2C is a HBV host factor.
From: A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor
a Individual ORF overexpression in Huh-106 and HBV infection 3 days after transduction. Detection of HBV pgRNA by qRT-PCR 10 dpi. Results are expressed as means +/− SEM relative pgRNA expression (%) compared to ctrl (set as 100%) from 8 independent experiments (n = 21). b, c siRNA transfection of HepG2-NTCP cells. b mRNA. Results are expressed as means +/− SEM relative expression compared to si ctrl (set to 1) from 4 independent experiments (n = 8). c HBV infection after silencing was detected by IF 10 dpi. Scale bars: 100 µm. d Production of CDKN2C knockout cell lines. CDKN2C expression was controlled by western blot for in HepG2-NTCP (ctrl) and KO-CDKN2C clones. e HBV infection of HepG2-NTCP, KO-CDKN2C clones, and Huh-106. HBV infection was assessed at 10 dpi by pgRNA qRT-PCR (black) and quantification of secreted HBeAg (white). Results are expressed as means +/− SEM % HBV infection compared to HepG2-NTCP (set as 100%) from 3 independent experiments (n = 9 for pgRNA and n = 12 for HBe CLIA). f Detection of endogenous CDKN2C expression in PHH from 7 donors. One experiment is shown. g Validation studies in PHH from 3 different donors transduced with ORF lentivirus for 3 days and infected with HBV. HBV markers (pgRNA, black; HBeAg, white) were detected 10 dpi. Results are expressed as means +/− SEM % HBV infection compared to ctrl (GFP) (set to 100%) from 3 independent experiments (n = 12 for pgRNA; n = 6 for HBeAg). h PHH from 3 donors were transduced with lentiviruses containing CDKN2C-targeting shRNA or non-targeting shRNA control (sh ctrl). Silencing efficacy was assessed by qRT-PCR. Results are expressed as means +/− SEM % gene expression compared to sh ctrl (set to 100%) from 3 independent experiments (n = 9). PHH were then infected with HBV and HBV infection was assessed by pgRNA qRT-PCR 8 dpi. Results are expressed as means +/− SEM relative pgRNA expression compared to sh ctrl (set to 100%) from 3 independent experiments (n = 9). **p < 0.01; ***p < 0.001 (two-tailed Mann–Whitney U test). Source data are provided as a Source Data file.
