Fig. 2: dRNAs suppress off-target editing by competing with sgRNAs for off-target sites. | Nature Communications

Fig. 2: dRNAs suppress off-target editing by competing with sgRNAs for off-target sites.

From: Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

Fig. 2

Representative gels of in vitro cleavage of PCR products containing either a FANCF-sgRNA2 OT1 or b the FANCF-sgRNA2 on-target site with either 150 or 450 fmoles of Cas9 FANCF-sgRNA2 RNP in the presence of variable amounts of the Cas9 FANCF-sgRNA2 OT1 dRNA1 complex. Fraction of uncut DNA determined by gel densitometry. Means of n = 3 replicates depicted by solid lines. For uncropped gels, see Supplementary Fig. 14. Source data are available in the Source Data file.

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